LauraLaphamUniversity of Maryland Center for Environmental Sciencelapham@umces.eduhttps://orcid.org/0000-0002-7045-1785JessicaBuser-Youngbuserj@oregonstate.eduAndrewThurberAndrew.Thurber@oregonstate.eduFredrickColwellrick.colwell@oregonstate.eduUS.DOE. > Office of Science > Subsurface Biogeochemical Research (SBR)fundingOrganization2021-02-09This data package contains geochemical and river water temperature data from OsmoSamplers deployed at East River, Colorado pump house. There are three data files. The first one contains sulfate (SO4) and chloride (Cl) concentrations from riverwater and hyporheic zone (sediment water interface (SWI), 4 cm below seafloor (cmbsf), and 12 (cmbsf)). The second file contains methane concentrations, methane stable carbon isotope ratios and water isotopes from hyporheic zone porewaters. And the third is from a temperature logger during the deployment. Files are provided as .xls or .csv. This research was performed as part of the SBR funded project “Quantifying Subsurface Biogeochemical Variability in a High Altitude Watershed During Winter Isolation” DE-SC0018328. The novel part of this dataset is that we quantify these geochemical parameters throughout the winter isolation period, which is difficult to measure, as well as within the hyporheic zone. The data files were updated 3/1/2021 because the column label for the water isotopes was incorrect. Also, the csv files were also updated since they were not formatted correctly.methanestable isotopestemporal subsettingbiogeochemistryCATEGORICAL:NONE

Buser-Young, J. et al., Hidden processes during seasonal isolation of a high-altitude watershed. Submitted to Frontiers in Earth Sciences, Feb 2021

This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.This study took place at the U.S. Department of Energy’s Biological and Environmental Research (DOE BER) station located at East River (ER) in Crested Butte, CO, USA. OsmoSamplers were deployed at near the SFA pumphouse, 38.922°N 106.951°W-106.951-106.95138.92238.9222017-11-012018-09-21LauraLaphamlapham@umces.eduhttps://orcid.org/0000-0002-7045-1785LauraLaphamUniversity of Maryland Center for Environmental Sciencelapham@umces.eduhttps://orcid.org/0000-0002-7045-1785Quantifying Subsurface Biogeochemical Variability in a High Altitude Watershed During Winter IsolationMethods will be published in Buser-Young, J. et al., Hidden processes during seasonal isolation of a high-altitude watershed. Submitted to Frontiers in Earth Sciences, Feb 2021. They are briefly described here.Step 1: four OsmoSamplers were placed in the river, with tubing inlets sampling the river water column, sediment water interface, 4 cm into the hyporheic zone, and 12 cm into the hyporheic zone. All OsmoSamplers contained copper small-bore tubing excluding the OsmoSampler inlet placed in the water column which contained Teflon tubing. The hyporheic sampling ports were supported along a PVC tube with 0.2 µm rhizon filters to exclude detritus or microbes that may alter the chemistry during long-term storage within the sample tubing.Recover OsmoSamplers 9 months later.Section tubing for river water (RW), sediment water interface (SWI), and hyporheic zones (HZ) samples into 4.5 m and 1.5 m lengths to obtain ~5 day resolution as calculated from the rate of sampling. Expel contents using a jewelry roller and store the fluid from the short section (~1.5 mL) in 2 mL microcentrifuge tubesOn the short section sample, measure sulfate and chloride concentrations via ion chromatography using AS40 analytical column and a conductivity detector (Dionex ICS1000) with 2% precision.Measure methane concentrations from fluids obtained from the large section (~2 mL) which was injected into a zero-air flushed, pre-combusted 10 mL glass serum vial, using caution not to contaminate the sample with air. Using a headspace equilibration technique, inject sample with ~8 mL of brine solution to displace an aliquot of the remaining headspace which can then be injected into a gas chromatograph with a flame ionization detector (SRI 8610C Multi Gas, 4 mL loop injection, MolSieve 13X column). Final dissolved methane concentrations were calculated by correcting for dilutions and using Henry’s Law. Precision was 2%.On the same sample from step 5 where headspace concentrations are above 100ppm methane, measure stable carbon isotope ratio for methane dissolved in water. Take an aliquot of this headspace and inject into a cavity ring down spectrometer (Picarro 2201i) for stable carbon isotope ratio of methane determination. Use two certified gas standards for calibration (Isometrics, 2500 ppm, -66.5 and -38.3‰), and precision was 0.5‰.Water isotopes (d18O and d2H) were also analyzed for every other OsmoSampler sample from SWI, 4 cm, and 12 cm using a GasBench® peripheral interfaced to an isotope ratio mass spectrometer (Thermo Delta V, precision of 0.1 and 1‰ for d18O and d2H, respectively)FredrickColwellOregon State Univ.rcolwell@coas.oregonstate.eduprincipalInvestigatorDOE:DE-SC0018328river_water_temperature_record.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetOsmoSampler_CH4_H2O_isotopes.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetOsmoSampler_Cl_SO4.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetOsmoSampler_CH4_H2O_isotopes_4cm_csv.csvapplication/vnd.ms-excelOsmoSampler_CH4_H2O_isotopes_12cm_csv.csvapplication/vnd.ms-excelOsmoSampler_CH4_H2O_isotopes_SWI_csv.csvapplication/vnd.ms-excelOsmoSampler_Cl_SO4_4cm_csv.csvapplication/vnd.ms-excelOsmoSampler_Cl_SO4_12cm_csv.csvapplication/vnd.ms-excelOsmoSampler_Cl_SO4_riverwater_csv.csvapplication/vnd.ms-excelOsmoSampler_Cl_SO4_SWI_csv.csvapplication/vnd.ms-excel